Retrieving a sequence


	If you do not already have the sequence to be searched on your computer, you will need to download it to your computer. If your computer has access to the Internet, the easiest route is to enter the accession number of the protein in question in the retrieval box in the main toolbar:


	If the web toolbar is not present in the toolbar, right-click in anywhere in the toolbar and in the pop-up menu check the “Web” option.

The accession number is usually reported as part of the name of the ‘protein hit’ (an example using Mascot is shown at right.
You enter the accession number as written here. The only exception is when you are using Entrez gene index numbers (gi numbers) then you have to write gi| in front of the accession number (e.g. gi|24642910). Click on the globe icon to the right in order to load the sequence.

Press the enter key or the ‘Get’ button to retrieve the sequence which will be displayed in the ‘Import ASCII file’ dialog box (below).
Note that if you click the drop-down arrow to the right of the edit box, a box will show the last 10 sequences retrieved.


	In most cases the file format will be recognized directly and the record will be split into: Name Sequence Accession number If this automatic parsing is not done, check here how to do it manually. Click the OK button to retrieve the sequence into GPMAW. NOTE: Accession numbers starting with O, P, Q are searched through the EBI server and the sequence records are retrieved in Swiss-Prot format. All other accession numbers are searched in the Entrez database (NCBI). The complete sequence record is saved on the annotation page. The Swiss-Prot format is recommended, as GPMAW is able to extract information on secondary modifications through the annotation dialog).

		Site last updated: April 14, 2024